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primary antibody against cx40  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology primary antibody against cx40
    Primary Antibody Against Cx40, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibody against cx40/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    primary antibody against cx40 - by Bioz Stars, 2026-02
    90/100 stars

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    (A) Representative en face images of eGFP in longitudinally opened carotids of <t>Cx40</t> +/eGFP mice. eGFP (green) is highly expressed in the straight portions of the vessel (upper panel) but not at the iliac bifurcation (lower panel). DAPI (blue). (B) Cx40 expression (green) after modification of shear stress by a vascular cast in Cx40 +/eGFP mice. Shown are the contralateral undisturbed vessel (control) and the regions upstream (LLSS), within (HLSS) and downstream of the cast (OSS). Evans Blue (red). DAPI (blue). (C) Quantification of (B); N=8. (D) eGFP after modification of shear stress by a vascular cast in Cx40 +/eGFP mice. Shown are the contralateral undisturbed vessel (control) and the regions upstream (LLSS), within (HLSS) and downstream of the cast (OSS). (E) Cx40 expression in bEnd.3 cells exposed to static, HLSS, LLSS, OSS conditions for 24 hours was assessed by real-time qPCR. N=5. (F) Representative images of Cx40 expression (green) in bEnd.3 cells exposed to HLSS, LLSS, OSS for 24 hours. Scale bar represents 50 μm for in (A), (B) and (D), and 40 μm in (F).
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    (A) Representative en face images of eGFP in longitudinally opened carotids of <t>Cx40</t> +/eGFP mice. eGFP (green) is highly expressed in the straight portions of the vessel (upper panel) but not at the iliac bifurcation (lower panel). DAPI (blue). (B) Cx40 expression (green) after modification of shear stress by a vascular cast in Cx40 +/eGFP mice. Shown are the contralateral undisturbed vessel (control) and the regions upstream (LLSS), within (HLSS) and downstream of the cast (OSS). Evans Blue (red). DAPI (blue). (C) Quantification of (B); N=8. (D) eGFP after modification of shear stress by a vascular cast in Cx40 +/eGFP mice. Shown are the contralateral undisturbed vessel (control) and the regions upstream (LLSS), within (HLSS) and downstream of the cast (OSS). (E) Cx40 expression in bEnd.3 cells exposed to static, HLSS, LLSS, OSS conditions for 24 hours was assessed by real-time qPCR. N=5. (F) Representative images of Cx40 expression (green) in bEnd.3 cells exposed to HLSS, LLSS, OSS for 24 hours. Scale bar represents 50 μm for in (A), (B) and (D), and 40 μm in (F).
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    (A) Representative en face images of eGFP in longitudinally opened carotids of <t>Cx40</t> +/eGFP mice. eGFP (green) is highly expressed in the straight portions of the vessel (upper panel) but not at the iliac bifurcation (lower panel). DAPI (blue). (B) Cx40 expression (green) after modification of shear stress by a vascular cast in Cx40 +/eGFP mice. Shown are the contralateral undisturbed vessel (control) and the regions upstream (LLSS), within (HLSS) and downstream of the cast (OSS). Evans Blue (red). DAPI (blue). (C) Quantification of (B); N=8. (D) eGFP after modification of shear stress by a vascular cast in Cx40 +/eGFP mice. Shown are the contralateral undisturbed vessel (control) and the regions upstream (LLSS), within (HLSS) and downstream of the cast (OSS). (E) Cx40 expression in bEnd.3 cells exposed to static, HLSS, LLSS, OSS conditions for 24 hours was assessed by real-time qPCR. N=5. (F) Representative images of Cx40 expression (green) in bEnd.3 cells exposed to HLSS, LLSS, OSS for 24 hours. Scale bar represents 50 μm for in (A), (B) and (D), and 40 μm in (F).
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    (A) Representative en face images of eGFP in longitudinally opened carotids of <t>Cx40</t> +/eGFP mice. eGFP (green) is highly expressed in the straight portions of the vessel (upper panel) but not at the iliac bifurcation (lower panel). DAPI (blue). (B) Cx40 expression (green) after modification of shear stress by a vascular cast in Cx40 +/eGFP mice. Shown are the contralateral undisturbed vessel (control) and the regions upstream (LLSS), within (HLSS) and downstream of the cast (OSS). Evans Blue (red). DAPI (blue). (C) Quantification of (B); N=8. (D) eGFP after modification of shear stress by a vascular cast in Cx40 +/eGFP mice. Shown are the contralateral undisturbed vessel (control) and the regions upstream (LLSS), within (HLSS) and downstream of the cast (OSS). (E) Cx40 expression in bEnd.3 cells exposed to static, HLSS, LLSS, OSS conditions for 24 hours was assessed by real-time qPCR. N=5. (F) Representative images of Cx40 expression (green) in bEnd.3 cells exposed to HLSS, LLSS, OSS for 24 hours. Scale bar represents 50 μm for in (A), (B) and (D), and 40 μm in (F).
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    (A) Representative en face images of eGFP in longitudinally opened carotids of <t>Cx40</t> +/eGFP mice. eGFP (green) is highly expressed in the straight portions of the vessel (upper panel) but not at the iliac bifurcation (lower panel). DAPI (blue). (B) Cx40 expression (green) after modification of shear stress by a vascular cast in Cx40 +/eGFP mice. Shown are the contralateral undisturbed vessel (control) and the regions upstream (LLSS), within (HLSS) and downstream of the cast (OSS). Evans Blue (red). DAPI (blue). (C) Quantification of (B); N=8. (D) eGFP after modification of shear stress by a vascular cast in Cx40 +/eGFP mice. Shown are the contralateral undisturbed vessel (control) and the regions upstream (LLSS), within (HLSS) and downstream of the cast (OSS). (E) Cx40 expression in bEnd.3 cells exposed to static, HLSS, LLSS, OSS conditions for 24 hours was assessed by real-time qPCR. N=5. (F) Representative images of Cx40 expression (green) in bEnd.3 cells exposed to HLSS, LLSS, OSS for 24 hours. Scale bar represents 50 μm for in (A), (B) and (D), and 40 μm in (F).
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    (A) Representative en face images of eGFP in longitudinally opened carotids of <t>Cx40</t> +/eGFP mice. eGFP (green) is highly expressed in the straight portions of the vessel (upper panel) but not at the iliac bifurcation (lower panel). DAPI (blue). (B) Cx40 expression (green) after modification of shear stress by a vascular cast in Cx40 +/eGFP mice. Shown are the contralateral undisturbed vessel (control) and the regions upstream (LLSS), within (HLSS) and downstream of the cast (OSS). Evans Blue (red). DAPI (blue). (C) Quantification of (B); N=8. (D) eGFP after modification of shear stress by a vascular cast in Cx40 +/eGFP mice. Shown are the contralateral undisturbed vessel (control) and the regions upstream (LLSS), within (HLSS) and downstream of the cast (OSS). (E) Cx40 expression in bEnd.3 cells exposed to static, HLSS, LLSS, OSS conditions for 24 hours was assessed by real-time qPCR. N=5. (F) Representative images of Cx40 expression (green) in bEnd.3 cells exposed to HLSS, LLSS, OSS for 24 hours. Scale bar represents 50 μm for in (A), (B) and (D), and 40 μm in (F).
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    (A) Representative en face images of eGFP in longitudinally opened carotids of <t>Cx40</t> +/eGFP mice. eGFP (green) is highly expressed in the straight portions of the vessel (upper panel) but not at the iliac bifurcation (lower panel). DAPI (blue). (B) Cx40 expression (green) after modification of shear stress by a vascular cast in Cx40 +/eGFP mice. Shown are the contralateral undisturbed vessel (control) and the regions upstream (LLSS), within (HLSS) and downstream of the cast (OSS). Evans Blue (red). DAPI (blue). (C) Quantification of (B); N=8. (D) eGFP after modification of shear stress by a vascular cast in Cx40 +/eGFP mice. Shown are the contralateral undisturbed vessel (control) and the regions upstream (LLSS), within (HLSS) and downstream of the cast (OSS). (E) Cx40 expression in bEnd.3 cells exposed to static, HLSS, LLSS, OSS conditions for 24 hours was assessed by real-time qPCR. N=5. (F) Representative images of Cx40 expression (green) in bEnd.3 cells exposed to HLSS, LLSS, OSS for 24 hours. Scale bar represents 50 μm for in (A), (B) and (D), and 40 μm in (F).
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    Image Search Results


    (A) Representative en face images of eGFP in longitudinally opened carotids of Cx40 +/eGFP mice. eGFP (green) is highly expressed in the straight portions of the vessel (upper panel) but not at the iliac bifurcation (lower panel). DAPI (blue). (B) Cx40 expression (green) after modification of shear stress by a vascular cast in Cx40 +/eGFP mice. Shown are the contralateral undisturbed vessel (control) and the regions upstream (LLSS), within (HLSS) and downstream of the cast (OSS). Evans Blue (red). DAPI (blue). (C) Quantification of (B); N=8. (D) eGFP after modification of shear stress by a vascular cast in Cx40 +/eGFP mice. Shown are the contralateral undisturbed vessel (control) and the regions upstream (LLSS), within (HLSS) and downstream of the cast (OSS). (E) Cx40 expression in bEnd.3 cells exposed to static, HLSS, LLSS, OSS conditions for 24 hours was assessed by real-time qPCR. N=5. (F) Representative images of Cx40 expression (green) in bEnd.3 cells exposed to HLSS, LLSS, OSS for 24 hours. Scale bar represents 50 μm for in (A), (B) and (D), and 40 μm in (F).

    Journal: Oncotarget

    Article Title: Connexin40 controls endothelial activation by dampening NFκB activation

    doi: 10.18632/oncotarget.16438

    Figure Lengend Snippet: (A) Representative en face images of eGFP in longitudinally opened carotids of Cx40 +/eGFP mice. eGFP (green) is highly expressed in the straight portions of the vessel (upper panel) but not at the iliac bifurcation (lower panel). DAPI (blue). (B) Cx40 expression (green) after modification of shear stress by a vascular cast in Cx40 +/eGFP mice. Shown are the contralateral undisturbed vessel (control) and the regions upstream (LLSS), within (HLSS) and downstream of the cast (OSS). Evans Blue (red). DAPI (blue). (C) Quantification of (B); N=8. (D) eGFP after modification of shear stress by a vascular cast in Cx40 +/eGFP mice. Shown are the contralateral undisturbed vessel (control) and the regions upstream (LLSS), within (HLSS) and downstream of the cast (OSS). (E) Cx40 expression in bEnd.3 cells exposed to static, HLSS, LLSS, OSS conditions for 24 hours was assessed by real-time qPCR. N=5. (F) Representative images of Cx40 expression (green) in bEnd.3 cells exposed to HLSS, LLSS, OSS for 24 hours. Scale bar represents 50 μm for in (A), (B) and (D), and 40 μm in (F).

    Article Snippet: Subsequently, primary antibodies against Cx40 (Cx40-A; Alpha-Diagnostics lot #175455A8.6; 1/200), Cx37 (Cx37A11-A; Alpha-Diagnostics lot #175859A5-L; 1/50), NFκB (NFκB p65 (A) sc-109; Santa Cruz; 1/100) or Phospho-NFκB (Phospho-NFκB (S536)(93H1); Cell Signaling; 1/100) in blocking solution were applied overnight at 4°C.

    Techniques: Expressing, Modification, Shear, Control

    (A) Cross-linking experiment with Cx40CT and peptides. Binding of IκBα-like or IκBα(5-16) peptides to Cx40CT was assessed after incubation with the chemical cross-linker BS 3 . All lanes show a band at ~16-17 kDa representing Cx40CT (arrow). The first and third lanes of the panel show an additional band at ~17-18kDa (arrow head), which is absent in lane 2, where the peptide is missing. (B) Representative en face images of PLA (out of 3 experiments) performed with antibodies targeting Cx40 and IκBα on rat carotid endothelium. Close proximity of Cx40 and IκBα (red) is observed in the intracellular compartment as well as at cell-cell contacts (left panel). Control assays revealed that the red staining observed was no longer observed after omitting either the Cx40 or the IκBα antibody from the PLA (right panel). Cx37 staining (green) is used to highlight the intercellular gap junctions. DAPI (blue). Scale bar represents 20 μm.

    Journal: Oncotarget

    Article Title: Connexin40 controls endothelial activation by dampening NFκB activation

    doi: 10.18632/oncotarget.16438

    Figure Lengend Snippet: (A) Cross-linking experiment with Cx40CT and peptides. Binding of IκBα-like or IκBα(5-16) peptides to Cx40CT was assessed after incubation with the chemical cross-linker BS 3 . All lanes show a band at ~16-17 kDa representing Cx40CT (arrow). The first and third lanes of the panel show an additional band at ~17-18kDa (arrow head), which is absent in lane 2, where the peptide is missing. (B) Representative en face images of PLA (out of 3 experiments) performed with antibodies targeting Cx40 and IκBα on rat carotid endothelium. Close proximity of Cx40 and IκBα (red) is observed in the intracellular compartment as well as at cell-cell contacts (left panel). Control assays revealed that the red staining observed was no longer observed after omitting either the Cx40 or the IκBα antibody from the PLA (right panel). Cx37 staining (green) is used to highlight the intercellular gap junctions. DAPI (blue). Scale bar represents 20 μm.

    Article Snippet: Subsequently, primary antibodies against Cx40 (Cx40-A; Alpha-Diagnostics lot #175455A8.6; 1/200), Cx37 (Cx37A11-A; Alpha-Diagnostics lot #175859A5-L; 1/50), NFκB (NFκB p65 (A) sc-109; Santa Cruz; 1/100) or Phospho-NFκB (Phospho-NFκB (S536)(93H1); Cell Signaling; 1/100) in blocking solution were applied overnight at 4°C.

    Techniques: Binding Assay, Incubation, Control, Staining

    (A) Lysates of bEnd.3 cells incubated or not with 10 ng/ml TNFα for 10, 15, 30 or 60 min were immunoblotted against NFκB, Cx40, IκBα, Phospho-IκBα or GAPDH. Whereas expression levels of NFκB, Cx40, and GAPDH were not affected by short-term stimulation with TNFα, the treatment induced phosphorylation and degradation of IκBα. (B) Quantification of (A) under control conditions or after incubation with 10 ng/ml TNFα for 15 min. N=3. (C) Expression of Cx40 (left panels) or NFκB (right panels; both in green) in control bEnd.3 cells and after 15 min stimulation with 10 ng/ml TNFα treated or not with siRNA for Cx40 or NT-siRNA. Note that in ECs in which Cx40 was silenced with siRNA (lower panels), NFκB translocation to the nucleus was enhanced after stimulation with TNFα. DAPI (blue). Scale bar represents 15 μm. (D) Cx40 expression in bEnd.3 cells exposed to Cx40 siRNA or NT-siRNA was assessed by real-time qPCR. N=3. (E) Expression of Cx40 (left) or Phospho-IκBα (right) in control bEnd.3 cells and after 15 min stimulation with 10 ng/ml TNFα treated with siRNA for Cx40 or NT-siRNA. N=3.

    Journal: Oncotarget

    Article Title: Connexin40 controls endothelial activation by dampening NFκB activation

    doi: 10.18632/oncotarget.16438

    Figure Lengend Snippet: (A) Lysates of bEnd.3 cells incubated or not with 10 ng/ml TNFα for 10, 15, 30 or 60 min were immunoblotted against NFκB, Cx40, IκBα, Phospho-IκBα or GAPDH. Whereas expression levels of NFκB, Cx40, and GAPDH were not affected by short-term stimulation with TNFα, the treatment induced phosphorylation and degradation of IκBα. (B) Quantification of (A) under control conditions or after incubation with 10 ng/ml TNFα for 15 min. N=3. (C) Expression of Cx40 (left panels) or NFκB (right panels; both in green) in control bEnd.3 cells and after 15 min stimulation with 10 ng/ml TNFα treated or not with siRNA for Cx40 or NT-siRNA. Note that in ECs in which Cx40 was silenced with siRNA (lower panels), NFκB translocation to the nucleus was enhanced after stimulation with TNFα. DAPI (blue). Scale bar represents 15 μm. (D) Cx40 expression in bEnd.3 cells exposed to Cx40 siRNA or NT-siRNA was assessed by real-time qPCR. N=3. (E) Expression of Cx40 (left) or Phospho-IκBα (right) in control bEnd.3 cells and after 15 min stimulation with 10 ng/ml TNFα treated with siRNA for Cx40 or NT-siRNA. N=3.

    Article Snippet: Subsequently, primary antibodies against Cx40 (Cx40-A; Alpha-Diagnostics lot #175455A8.6; 1/200), Cx37 (Cx37A11-A; Alpha-Diagnostics lot #175859A5-L; 1/50), NFκB (NFκB p65 (A) sc-109; Santa Cruz; 1/100) or Phospho-NFκB (Phospho-NFκB (S536)(93H1); Cell Signaling; 1/100) in blocking solution were applied overnight at 4°C.

    Techniques: Incubation, Expressing, Phospho-proteomics, Control, Translocation Assay

    (A) Cx40 immunostaining (green) in communication-incompetent HeLa cells (left panel) and in HeLa cells stably transfected with Cx40 (right panel). DAPI (blue). (B) Intercellular communication was measured by Lucifer Yellow microinjection during 3 min. Images are representative examples of Lucifer Yellow diffusion in parental HeLa cells (upper panel, N=6) and in HeLa cells stably transfected with Cx40 (lower panel, N=10). Asterisks indicate the microinjected cells. (C) Western blots (upper panel) showing the induction of Phospho-NFκB after 5 min stimulation with 20 ng/ml TNFα (+) as compared to control conditions (-) in parental HeLa cells and in HeLa cells stably transfected with Cx40. Expression of Cx40 reduced TNFα-induced NFκB phosphorylation (lower panel). N=3. (D) Cx40 immunostaining (green) in communication-incompetent HeLa cells transiently transfected with full-length Cx40 (left panel) or with Cx40CT (right panel). DAPI (blue). (E) Lysates of parental HeLa cells (lane 1) or transiently transfected with Cx40 (lanes 2 and 4) or Cx40CT (lanes 3 and 5) or stably transfected with Cx40 (lane 6) were immunoblotted against Cx40 and GAPDH. (F) Intercellular communication was measured by Lucifer Yellow microinjection during 5 min. Images are representative examples of Lucifer Yellow diffusion in HeLa cells transiently transfected with Cx40 (upper panel, N=8) or Cx40CT (lower panel, N=6). Asterisks indicate the microinjected cells. (G) Induction of Phospho-NFκB after 5 min stimulation with 20 ng/ml TNFα in HeLa cells transiently transfected with Cx40 or Cx40CT. Expression of Cx40 or Cx40CT revealed a similar protection against TNFα-induced NFκB phosphorylation. N=3. Scale bar represents 10 μm in (A) and (D), and 15 μm in (B) and (F).

    Journal: Oncotarget

    Article Title: Connexin40 controls endothelial activation by dampening NFκB activation

    doi: 10.18632/oncotarget.16438

    Figure Lengend Snippet: (A) Cx40 immunostaining (green) in communication-incompetent HeLa cells (left panel) and in HeLa cells stably transfected with Cx40 (right panel). DAPI (blue). (B) Intercellular communication was measured by Lucifer Yellow microinjection during 3 min. Images are representative examples of Lucifer Yellow diffusion in parental HeLa cells (upper panel, N=6) and in HeLa cells stably transfected with Cx40 (lower panel, N=10). Asterisks indicate the microinjected cells. (C) Western blots (upper panel) showing the induction of Phospho-NFκB after 5 min stimulation with 20 ng/ml TNFα (+) as compared to control conditions (-) in parental HeLa cells and in HeLa cells stably transfected with Cx40. Expression of Cx40 reduced TNFα-induced NFκB phosphorylation (lower panel). N=3. (D) Cx40 immunostaining (green) in communication-incompetent HeLa cells transiently transfected with full-length Cx40 (left panel) or with Cx40CT (right panel). DAPI (blue). (E) Lysates of parental HeLa cells (lane 1) or transiently transfected with Cx40 (lanes 2 and 4) or Cx40CT (lanes 3 and 5) or stably transfected with Cx40 (lane 6) were immunoblotted against Cx40 and GAPDH. (F) Intercellular communication was measured by Lucifer Yellow microinjection during 5 min. Images are representative examples of Lucifer Yellow diffusion in HeLa cells transiently transfected with Cx40 (upper panel, N=8) or Cx40CT (lower panel, N=6). Asterisks indicate the microinjected cells. (G) Induction of Phospho-NFκB after 5 min stimulation with 20 ng/ml TNFα in HeLa cells transiently transfected with Cx40 or Cx40CT. Expression of Cx40 or Cx40CT revealed a similar protection against TNFα-induced NFκB phosphorylation. N=3. Scale bar represents 10 μm in (A) and (D), and 15 μm in (B) and (F).

    Article Snippet: Subsequently, primary antibodies against Cx40 (Cx40-A; Alpha-Diagnostics lot #175455A8.6; 1/200), Cx37 (Cx37A11-A; Alpha-Diagnostics lot #175859A5-L; 1/50), NFκB (NFκB p65 (A) sc-109; Santa Cruz; 1/100) or Phospho-NFκB (Phospho-NFκB (S536)(93H1); Cell Signaling; 1/100) in blocking solution were applied overnight at 4°C.

    Techniques: Immunostaining, Stable Transfection, Transfection, Microinjection, Diffusion-based Assay, Western Blot, Control, Expressing, Phospho-proteomics

    Representative images of SudanIV stainings are shown for the 3 flow regions of casted vessels (LLSS, HLSS, OSS) from Cx40 fl/fl Apoe -/- (A) and Tie2-cre Tg Cx40 fl/fl Apoe -/- (B, C) mice after 6 weeks of high-cholesterol diet. Intimal thickening was present in regions subjected to LLSS and OSS in Cx40 fl/fl Apoe -/- mice (A). Intimal thickening in response to LLSS and OSS was increased in Tie2-cre Tg Cx40 fl/fl Apoe -/- mice (B). The increased atherosclerotic response caused in half of the Tie2-cre Tg Cx40 fl/fl Apoe -/- mice a complete occlusion of the LLSS area that gave rise to atherosclerotic lesions within the cast (C). N=6-8 animals per group. Scale bar = 200 μm. (D, E) En face immunostaining for NFκB (in green) in carotid arteries of Cx40 fl/fl Apoe -/- (D) and Tie2-cre Tg Cx40 fl/fl Apoe -/- (E) mice. Note that NFκB signal was mostly cytoplasmic in Cx40 fl/fl Apoe -/- mice and more frequently localized to the nucleus in Tie2-cre Tg Cx40 fl/fl Apoe -/- mice. DAPI (blue). Scale bar represents 10 μm.

    Journal: Oncotarget

    Article Title: Connexin40 controls endothelial activation by dampening NFκB activation

    doi: 10.18632/oncotarget.16438

    Figure Lengend Snippet: Representative images of SudanIV stainings are shown for the 3 flow regions of casted vessels (LLSS, HLSS, OSS) from Cx40 fl/fl Apoe -/- (A) and Tie2-cre Tg Cx40 fl/fl Apoe -/- (B, C) mice after 6 weeks of high-cholesterol diet. Intimal thickening was present in regions subjected to LLSS and OSS in Cx40 fl/fl Apoe -/- mice (A). Intimal thickening in response to LLSS and OSS was increased in Tie2-cre Tg Cx40 fl/fl Apoe -/- mice (B). The increased atherosclerotic response caused in half of the Tie2-cre Tg Cx40 fl/fl Apoe -/- mice a complete occlusion of the LLSS area that gave rise to atherosclerotic lesions within the cast (C). N=6-8 animals per group. Scale bar = 200 μm. (D, E) En face immunostaining for NFκB (in green) in carotid arteries of Cx40 fl/fl Apoe -/- (D) and Tie2-cre Tg Cx40 fl/fl Apoe -/- (E) mice. Note that NFκB signal was mostly cytoplasmic in Cx40 fl/fl Apoe -/- mice and more frequently localized to the nucleus in Tie2-cre Tg Cx40 fl/fl Apoe -/- mice. DAPI (blue). Scale bar represents 10 μm.

    Article Snippet: Subsequently, primary antibodies against Cx40 (Cx40-A; Alpha-Diagnostics lot #175455A8.6; 1/200), Cx37 (Cx37A11-A; Alpha-Diagnostics lot #175859A5-L; 1/50), NFκB (NFκB p65 (A) sc-109; Santa Cruz; 1/100) or Phospho-NFκB (Phospho-NFκB (S536)(93H1); Cell Signaling; 1/100) in blocking solution were applied overnight at 4°C.

    Techniques: Immunostaining